Molecular crowding effects on protein stability

被引：48

作者：

Despa, Florin

Orgill, Dennis P.

Lee, Raphael C.

机构：

[1] Univ Chicago, Dept Surg, Chicago, IL 60637 USA

[2] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Surg, Boston, MA 02115 USA

来源：

CELL INJURY: MECHANISMS, RESPONSES, AND REPAIR | 2005年 / 1066卷

关键词：

crowding effects; protein denaturation; thermal injury;

D O I：

10.1196/annals.1363.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The volume fraction occupied by the dry matter of the cell can be as large as 40%, of which more than half (-60%) are proteins. Thus, cellular proteins and protein assemblies occupy a large volume that can have a profound effect on their own native-state stabilities and on their unfolding/refolding rates. In addition, macromolecular crowding can change the properties of a significant fraction of the water in the cell. We review features of the molecular crowding effect which are relevant for describing the microscopic mechanism of thermal injuries.

引用

页码：54 / 66

页数：13

共 22 条

[1]

ALBERTS B, 1983, MOL BIOL CELL, P111

[2] Macromolecular crowding as a cell volume sensor [J].