veratryl alcohol;
manganese-peroxidase;
aromatic ring opening;
white-rot fungi;
Lentinus edodes;
D O I:
10.1016/0168-1656(96)01513-1
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
A manganese-peroxidase from Lentinus edodes solid-state cultures was purified and its ability to bring about the oxidation of veratryl alcohol was investigated. Such investigations were carried out in the absence and in the presence of reduced glutathione (GSH), as a thiol-donor. Two different biodegradative pathways were identified, in the former case leading to aromatic ring cleavage, side chain oxidation and dimerization, while in the latter case veratraldehyde was recovered as the only metabolite.