Direct bioautography-thin-layer chromatography of flumequine and doxycycline in milk

被引:10
作者
Choma, I
Choma, A
Staszczuk, K
机构
[1] Marie Curie Sklodowska Univ, Dept Chem Phys & Physicochem Methods Separat, PL-20031 Lublin, Poland
[2] Marie Curie Sklodowska Univ, Dept Gen Microbiol, PL-20033 Lublin, Poland
关键词
thin-layer chromatography; direct bioautography; milk; flumequine; doxycycline;
D O I
10.1556/JPC.15.2002.3.5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Thin-layer chromatography combined with bioautography (TLC-B) is a screening technique which combines TLC with microbiological detection and results in enhanced sensitivity. In the work discussed in this paper TLC-B was used to detect two veterinary antibiotics, doxycycline and flumequine, in milk. The sensitivity of bioautography was compared with that of UV detection. All procedures, i.e. clean-up, concentration, separation, and detection, were performed on the same TLC plate.
引用
收藏
页码:187 / 191
页数:5
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