Evidence for expression and function of angiotensin II receptor type 1 in pulmonary epithelial cells

被引：4

作者：

Ashry, Omaima ^{[1
,2
]}

Schnecko, Anja ^{[1
]}

Clauss, Wolfgang G. ^{[1
]}

Fronius, Martin ^{[1
]}

机构：

[1] Univ Giessen, Inst Anim Physiol, D-35390 Giessen, Germany

[2] Natl Ctr Radiat Res & Technol, Radiat Biol Dept, Cairo, Egypt

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2014年 / 195卷

关键词：

Pulmonary epithelium; Pulmonary ion transport; Ussing chamber; Angiotensin II; Angiotensin II receptor; Xenopus laevis; SYSTEMS;

D O I：

10.1016/j.resp.2014.02.001

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

There is a growing evidence that the peptide hormone angiotensin II (ANGII) can act as an auto-/paracrine mediator to regulate epithelial ion transport processes. The present study focused on the impact of ANGII on transepithelial ion transport in pulmonary epithelia. Transcripts for the ANGII receptor type 1 (ATRi) were detected in lungs of Xenopus laevis and H441 cells (human pulmonary epithelial cell line). Native Xenopus lung preparations were used for Ussing chamber recordings and apically applied ANGII (10 mu M) induced a significant increase of short-circuit current (Isc: 8 +/- 2%, n =13). Pre-incubation with losartan (LOS), an antagonist of ATRi prevented the effect of ANGII on I-Sc. Transcripts for ATR(1) in Xenopus lungs and H441 cells were detected and an increase of I-sc was observed by ANGII in native Xenopus lung epithelia. This indicates that ANGII is a potential auto-/paracrine mediator for ion transport regulation in pulmonary epithelia. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：37 / 40

页数：4

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