High-throughput RNA extraction from plant samples based on homogenisation by reciprocal shaking in the presence of a mixture of sand and glass beads

被引：73

作者：

Eggermont, K ^{[1
]}

Goderis, IJ ^{[1
]}

Broekaert, WF ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1996年 / 14卷 / 03期

关键词：

Arabidopsis thaliana; glass beads; reciprocal shaking; RNA extraction; sand;

D O I：

10.1007/BF02671663

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here a reliable high-throughput method for extraction of RNA from fresh or frozen plant tissue that obviates laborious and time-consuming homogenisation by mortar and pestle. The method is based on homogenisation by high-speed reciprocal shaking in presence of a mixture of inexpensive abrasive materials; i.e., quartz sand and glass beads. After homogenisation, the method follows a standard procedure for RNA extraction by phenol/LiCl. Yield and quality of RNA obtained by homogenisation with the sand/glass bead mix are identical to those obtained by mortar and pestle.

引用

页码：273 / 279

页数：7

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