Detection of circulating Leishmania chagasi DNA for the non-invasive diagnosis of human infection

被引：18

作者：

Disch, J ^{[1
]}

Maciel, FC ^{[1
]}

de Oliveira, MC ^{[1
]}

Orsini, M ^{[1
]}

Rabello, A ^{[1
]}

机构：

[1] Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Pesquisas Clin, BR-30190002 Belo Horizonte, MG, Brazil

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2003年 / 97卷 / 04期

关键词：

visceral leishmaniasis; Leishmania (Leishmania) chagasi; polymerase chain reaction; Brazil;

D O I：

10.1016/S0035-9203(03)90066-6

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A polymerase chain reaction (PCR) assay for the detection of Leishmania spp. DNA in peripheral blood was optimized and evaluated for the diagnosis of human visceral leishmaniasis (VL) in Brazil during May 2001 to December 2002. Optimization of the technique resulted in a detection limit of 1.65 fg of purified L. (L.) chagasi DNA, equivalent to 1.65 X 10(-2) parasites. Leishmania DNA was detected in the blood of 48 of 53 patients with parasitologically-confirmed VL, which corresponds to a sensitivity of 91%. No DNA was detected in the peripheral blood of 15 healthy, non-exposed volunteers, giving a specificity of 100%. We conclude that detection of parasite DNA in peripheral blood offers a non-invasive, sensitive and rapid method for the detection of VL caused by L. (L.) chagasi.

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收藏

页码：391 / 395

页数：5

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