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The pathogenesis of Newcastle disease: A comparison of selected Newcastle disease virus wild-type strains and their infectious clones

被引：45
作者：
Wakamatsu, Nobuko
King, Daniel J.
Seal, Bruce S.
Samal, Siba K.
Brown, Corrie C.
机构：
[1] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA
[2] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA 30605 USA
[3] USDA ARS, Poultry Microbiol Safety Unit, Russell Res Ctr, Athens, GA 30605 USA
[4] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA
来源：
VIROLOGY | 2006年 / 353卷 / 02期
关键词：
fusion protein; hemagglutinin-neuraminidase protein; immunohistochemistry; in situ hybridization; phosphoprotein; reverse genetics; avian paramyxovirus; veterinary virology; emerging disease;
D O I：
10.1016/j.virol.2006.06.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The effect of mutations of Newcastle disease virus (NDV) fusion (F) gene, hemagglutinin-neuraminidase (HN) gene, and phosphoprotein (P) gene and HN chimeras between the virulent Beaudette C and low virulence LaSota strains on pathogenesis and pathogenicity was examined in fully susceptible chickens. A virulent F cleavage site motif within a LaSota backbone increased pathogenicity and severity of clinical disease. A LaSota HN within a Beaudette C backbone decreased pathogenicity indices and disease severity. A Beaudette C HN within a LaSota backbone did not change either pathogenicity indices or severity of disease in chickens. Loss of glycosylation at site 4 of the HN or modified P gene of Beaudette C decreased pathogenicity indices and caused no overt clinicopathologic disease in chickens. Both pathogenicity indices and clinicopathologic examination demonstrated that the F, RN, and P genes of NDV collectively or individually can contribute to viral virulence. (c) 2006 Elsevier Inc. All rights reserved.
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收藏
页码：333 / 343
页数：11
相关论文
共 41 条
[1]   Tissue tropism in the chicken embryo of non-virulent and virulent Newcastle diseases strains that express green fluorescence protein [J].
Al-Garib, SO ;
Gielkens, ALJ ;
Gruys, E ;
Peeters, BPH ;
Koch, G .
AVIAN PATHOLOGY, 2003, 32 (06) :591-596
[2]   Newcastle disease [J].
Alexander, D .
BRITISH POULTRY SCIENCE, 2001, 42 (01) :5-22
[3]  
Alexander D.J., 1998, A laboratory manual for the isolation and identification of avian pathogens, V4th, P156
[4]   NEWCASTLE-DISEASE IN COUNTRIES OF THE EUROPEAN UNION [J].
ALEXANDER, DJ .
AVIAN PATHOLOGY, 1995, 24 (01) :3-10
[5]  
Alexander DJ, 2003, DIS POULTRY, P63
[6]   Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence [J].
Brown, C ;
King, DJ ;
Seal, BS .
VETERINARY PATHOLOGY, 1999, 36 (02) :125-132
[7]   MOLECULAR-CLONING OF COMPLEMENTARY-DNA TO NEWCASTLE-DISEASE VIRUS, AND NUCLEOTIDE-SEQUENCE ANALYSIS OF THE JUNCTION BETWEEN THE GENES ENCODING THE HEMAGGLUTININ NEURAMINIDASE AND THE LARGE PROTEIN [J].
CHAMBERS, P ;
MILLAR, NS ;
BINGHAM, RW ;
EMMERSON, PT .
JOURNAL OF GENERAL VIROLOGY, 1986, 67 :475-486
[8]   Complete nucleotide sequence of Newcastle disease virus:: evidence for the existence of a new genus within the subfamily Paramyxovirinae [J].
de Leeuw, O ;
Peeters, B .
JOURNAL OF GENERAL VIROLOGY, 1999, 80 :131-136
[9]   Virulence of Newcastle diseasevirus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein [J].
de Leeuw, OS ;
Koch, G ;
Hartog, L ;
Ravenshorst, N ;
Peeters, BPH .
JOURNAL OF GENERAL VIROLOGY, 2005, 86 :1759-1769
[10]   Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain [J].
de Leeuw, OS ;
Hartog, L ;
Koch, G ;
Peeters, BPH .
JOURNAL OF GENERAL VIROLOGY, 2003, 84 :475-484
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