IgE enhances mouse mast cell Fc epsilon RI expression in vitro and in vivo: Evidence for a novel amplification mechanism in IgE-dependent reactions

被引:372
作者
Yamaguchi, M
Lantz, CS
Oettgen, HC
Katona, IM
Fleming, T
Miyajima, I
Kinet, JP
Galli, SJ
机构
[1] BETH ISRAEL DEACONESS MED CTR E,DIV EXPT PATHOL,DEPT PATHOL,BOSTON,MA 02215
[2] HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02215
[3] CHILDRENS HOSP,DIV IMMUNOL,BOSTON,MA 02215
[4] UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT PEDIAT,BETHESDA,MD 20814
[5] UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT MED,BETHESDA,MD 20814
关键词
D O I
10.1084/jem.185.4.663
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The binding of immunoglobulin E (IgE) to high affinity IgE receptors (Fc epsilon RI) expressed on the surface of mast cells primes these cells to secrete, upon subsequent exposure to specific antigen, a panel of proinflammatory mediators, which includes cytokines that can also have immunoregulatory activities. This IgE- and antigen-specific mast cell activation and mediator production is thought to be critical to the pathogenesis of allergic disorders, such as anaphylaxis and asthma, and also contributes to host defense against parasites. We now report that exposure to IgE results in a striking (up to 32-fold) upregulation of surface expression of Fc epsilon RI on mouse mast cells in vitro or in vivo. Moreover, baseline levels of Fc epsilon RI expression on peritoneal mast cells from genetically IgE-deficient (IgE -/-) mice are dramatically reduced (by similar to 83%) compared with those on cells from the corresponding normal mice. In vitro studies indicate that the IgE-dependent upregulation of mouse mast cell Fc epsilon RI expression has two components: an early cycloheximide-insensitive phase, followed by a later and more sustained component that is highly sensitive to inhibition by cycloheximide. In turn, IgE-dependent upregulation of Fc epsilon RI expression significantly enhances the ability of mouse mast cells to release serotonin, interleukin-6 (IL-6), and IL-4 in response to challenge with IgE and specific antigen. The demonstration that IgE-dependent enhancement of mast cell Fc epsilon RI expression permits mast cells to respond to antigen challenge with increased production of proinflammatory and immunoregulatory mediators provides new insights into both the pathogenesis of allergic diseases and the regulation of protective host responses to parasites.
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页码:663 / 672
页数:10
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