Induction of micronuclei in Syrian hamster embryo cells: Comparison to results in the SHE cell transformation assay for national toxicology program test chemicals

被引：26

作者：

Gibson, DP ^{[1
]}

Brauninger, R ^{[1
]}

Shaffi, HS ^{[1
]}

Kerckaert, GA ^{[1
]}

LeBoeuf, RA ^{[1
]}

Isfort, RJ ^{[1
]}

Aardema, MJ ^{[1
]}

机构：

[1] CORNING HAZLETON,VIENNA,VA 22182

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 1997年 / 392卷 / 1-2期

关键词：

in vitro; micronucleus; cell transformation; Syrian hamster embryo cell;

D O I：

10.1016/S0165-1218(97)00045-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sixteen chemicals currently being tested in National Toxicology Program (NTP) carcinogenicity studies were evaluated in the Syrian hamster embryo (SHE) cell in vitro micronucleus assay. Results from these studies were compared to the results from the SHE cell transformation assay for the same chemicals The overall concordance between induction of micronuclei and transformation of SHE cells was 56%, which is far lower that the 93% concordance between these two tests reported previously by Fritzenschaf et al. (1993; Mutation Res. 319, 47-53). The difference between our results appears to be due to differences in the types of chemicals in the two studies. Overall, there is good agreement between the SHE cell micronucleus and transformation assays for mutagenic chemicals, but, as our study highlights, the SHE cell transformation assay has the added utility of detecting nonmutagenic carcinogens. The utility of a multi-endpoint assessment in SHE cells for carcinogen screening is discussed. (C) 1997 Elsevier Science B.V.

引用

收藏

页码：61 / 70

页数：10

相关论文

共 26 条

[1] The low pH Syrian hamster embryo (SHE) cell transformation assay: A revitalized role in carcinogen prediction [J].

Aardema, MJ ;

Isfort, RJ ;

Thompson, ED ;

LeBoeuf, RA .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1996, 356 (01) :5-9

[2] EVIDENCE FOR PROGRESSIVE NATURE OF NEOPLASTIC TRANSFORMATION INVITRO [J].

BARRETT, JC ;

TSO, POP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3761-3765

[3] MUTAGENICITY OF CHLOROPROPANOL IN A GENETIC SCREENING BATTERY [J].

BILES, RW ;

PIPER, CE .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1983, 3 (01) :27-33

[4] TESTING OF KNOWN CARCINOGENS AND NONCARCINOGENS IN THE SYRIAN-HAMSTER EMBRYO (SHE) MICRONUCLEUS TEST IN-VITRO - CORRELATIONS WITH IN-VIVO MICRONUCLEUS FORMATION AND CELL-TRANSFORMATION [J].

FRITZENSCHAF, H ;

KOHLPOTH, M ;

RUSCHE, B ;

SCHIFFMANN, D .

MUTATION RESEARCH, 1993, 319 (01) :47-53

[5] DETECTION OF ANEUPLOIDY-INDUCING CARCINOGENS IN THE SYRIAN-HAMSTER EMBRYO (SHE) CELL-TRANSFORMATION ASSAY [J].

GIBSON, DP ;

AARDEMA, MJ ;

KERCKAERT, GA ;

CARR, GJ ;

BRAUNINGER, RM ;

LEBOEUF, RA .

MUTATION RESEARCH-GENETIC TOXICOLOGY, 1995, 343 (01) :7-24

[6] MUTACHROMOSOMAL EFFECTS OF TERT-BUTYLHYDROQUINONE IN BONE-MARROW CELLS OF MICE [J].

GIRI, AK ;

SEN, S ;

TALUKDER, G ;

SHARMA, A .

FOOD AND CHEMICAL TOXICOLOGY, 1984, 22 (06) :459-460

[7] EVALUATION OF THE CARCINOGENIC AND GENOTOXIC POTENTIAL OF PRAZIQUANTEL IN THE SYRIAN-HAMSTER EMBRYO CELL-TRANSFORMATION ASSAY [J].

HERRERA, LA ;

OSTROSKYWEGMAN, P ;

MONTERO, R ;

ROJAS, E ;

GONSEBATT, ME ;

SCHIFFMANN, D .

MUTATION RESEARCH, 1994, 305 (02) :175-180

[8] THE INSECTICIDE BUPROFEZIN INDUCES MORPHOLOGICAL TRANSFORMATION AND KINETOCHORE-POSITIVE MICRONUCLEI IN CULTURED SYRIAN-HAMSTER EMBRYO CELLS IN THE ABSENCE OF DETECTABLE DNA-DAMAGE [J].

HERRERA, LA ;

OSTROSKYWEGMAN, P ;

SCHIFFMANN, D ;

CHEN, QY ;

ZIEGLERSKYLAKAKIS, K ;

ANDRAE, U .

MUTATION RESEARCH, 1993, 303 (03) :121-125

[9] Comparison of the standard and reduced pH Syrian Hamster Embryo (SHE) cell in vitro transformation assays in predicting the carcinogenic potential of chemicals [J].

Isfort, RJ ;

Kerckaert, GA ;

LeBoeuf, RA .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1996, 356 (01) :11-63

[10] Use of the Syrian hamster embryo cell transformation assay for carcinogenicity prediction of chemicals currently being tested by the national toxicology program in rodent bioassays [J].

Kerckaert, GA ;

Brauninger, R ;

LeBoeuf, RA ;

Isfort, RJ .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1996, 104 :1075-1084