Methods and reagents - Optimizing multiplex and LA-PCR with betaine

被引：33

作者：

Hengen, PN

机构：

[1] National Cancer Institute, Frederick Cancer R. and D. Center, Frederick

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1997年 / 22卷 / 06期

关键词：

D O I：

10.1016/S0968-0004(97)01069-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the use of additives fcr optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR). For details on how to partake in the newsgroup, see the accompanying box.

引用

页码：225 / 226

页数：2

共 8 条

[1] Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content [J].