Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis

被引：21

作者：

Gibson, David S.

Blelock, Sarah

Brockbank, Simon

Curry, Jim

Healy, Adrienne

McAllister, Catherine

Rooney, Madeleine E.

机构：

[1] Queens Univ Belfast, Arthritis Res Grp, Musculoskeletal Educ & Res Unit, Musgrave Pk Hosp, Belfast BT9 7BL, Antrim, North Ireland

[2] Queens Univ Belfast, Royal Victoria Hosp, Inst Clin Sci, Belfast BT9 7BL, Antrim, North Ireland

[3] Queens Univ Belfast, Ctr Peptide & Prot Engn, Sch Biol & Biochem, Ctr Med Biol, Belfast BT9 7BL, Antrim, North Ireland

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 08期

关键词：

juvenile idiopathic arthritis; biomarker; inflammation; synovial fluid; plasma; RECEPTOR-BETA-CHAIN; RHEUMATOID-ARTHRITIS; MASS-SPECTROMETRY; ENDOCHONDRAL OSSIFICATION; REACTIVE ARTHRITIS; EARLY PREDICTORS; PROTEIN; CARTILAGE; SERUM; DIFFERENTIATION;

D O I：

10.1021/pr060129t

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synovial fluid proteome in juvenile idiopathic arthritis was investigated to isolate joint-specific biomarkers that are expressed in patients displaying recurrent joint inflammation. To identify the synovial specific proteome, matched synovial fluid and plasma samples were subjected to protein separation by 2-dimension electrophoresis (2DE). Forty-three protein spots, overexpressed in the joint, were identified. Synovial fluids from children with single-event knee joint inflammation were then compared with a group with recurrent knee disease. Nine synovial specific proteins were significantly differentially expressed in the recurrent group. Proteolytic fragments of collagen X, fibrin beta-chain, and T-cell receptor alpha-region have been identified among this protein cluster. Putative biomarkers, overexpressed in the joint and differentially expressed in children with recurrent joint inflammation, have been identified. These proteins may play a significant role determining the pathological state within the chronically inflamed joint and influence disease progression in JIA. This is the first study of the synovial proteome in children.

引用

页码：1988 / 1995

页数：8

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