Matrix merging arrangements for the study protein dynamics by time-resolved step-scan Fourier transform infrared spectroscopy and multivariate curve resolution

被引：10

作者：

Ruckebusch, C

Duponchel, L

Huvenne, JP

Saurina, J

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

[2] USTL, LASIR, CNRS, UMR 8516, F-59655 Villeneuve Dascq, France

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 515卷 / 01期

关键词：

matrix melting arrangement; multivariate curve resolution; time-resolved FT-IR; protein dynamics; bacteriorhodopsin;

D O I：

10.1016/j.aca.2003.11.062

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we propose the construction of merging arrangements for combining the information from various runs as a powerful approach to improve the resolution. The bacteriorhodopsin (bR) photocycle has been chosen in this study as an example dealing with the protein dynamics monitored by means of time-resolved step-scan FT-IR spectroscopy. The possibilities of matrix merging are evaluated and results are compared with those from the analysis of individual and augmented matrices. As a conclusion, this strategy provides excellent results for the analysis of this type of time-resolved FT-IR data. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：183 / 190

页数：8

共 22 条

[1] On the rank deficiency and rank augmentation of the spectral measurement matrix [J].