Permeate from cheese whey ultrafiltration is a source of milk oligosaccharides

被引:121
作者
Barile, Daniela [1 ]
Tao, Nannan [2 ]
Lebrilla, Carlito B. [2 ]
Coisson, Jean-Daniel [3 ]
Arlorio, Marco [3 ]
German, J. Bruce [1 ]
机构
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Chem & Biochem, Sch Med, Davis, CA 95616 USA
[3] Univ Piemonte Orientale, Dipartimento Sci Chim Alimentari Farmaceut & Farm, I-28100 Novara, Italy
基金
美国国家卫生研究院;
关键词
SIALIC-ACID; GALACTO-OLIGOSACCHARIDES; BETA-GALACTOSIDASE; BOVINE-MILK; LACTOSE; COLOSTRUM; SIALYLOLIGOSACCHARIDES;
D O I
10.1016/j.idairyj.2009.03.008
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
Previously undescribed oligosaccharides in bovine cheese whey permeate were characterized by a combination of nanoelectrospray Fourier transform ion cyclotron resonance (nESI-FTICR) mass spectrometry and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry. Oligosaccharide composition was elucidated by collision-induced dissociation within the ICR cell. In addition to sialyllactose (the most abundant oligosaccharide, in bovine colostrum), we identified 14 other oligosaccharides, half of which have the same composition of human milk oligosaccharides. These oligosaccharides could potentially be used as additives in infant formula and products for the pharmaceutical industry. Because whey permeate is a by-product from the production of whey protein concentrate (WPC) and is readily available, it is an attractive source of oligosaccharides for potential application in human nutrition. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:524 / 530
页数:7
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