Genome-wide Analysis of Histone Methylation Reveals Chromatin State-Based Regulation of Gene Transcription and Function of Memory CD8+ T Cells

被引：228

作者：

Araki, Yasuto ^{[2
]}

Wang, Zhibin ^{[1
]}

Zang, Chongzhi ^{[6
]}

Wood, William H., III ^{[3
]}

Schones, Dustin ^{[1
]}

Cui, Kairong ^{[1
]}

Roh, Tae-Young ^{[1
]}

Lhotsky, Brad ^{[4
]}

Wersto, Robert P. ^{[5
]}

Peng, Weiqun ^{[6
]}

Becker, Kevin G. ^{[3
]}

Zhao, Keji ^{[1
]}

Weng, Nan-ping ^{[2
]}

机构：

[1] NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA

[2] NIA, Immunol Lab, NIH, Baltimore, MD 21224 USA

[3] NIA, DNA Array Unit, NIH, Baltimore, MD 21224 USA

[4] NIA, IT Sect, NIH, Baltimore, MD 21224 USA

[5] NIA, Flow Cytometry Unit, NIH, Baltimore, MD 21224 USA

[6] George Washington Univ, Dept Phys, Washington, DC 20052 USA

来源：

IMMUNITY | 2009年 / 30卷 / 06期

基金：

美国国家卫生研究院;

关键词：

IFN-GAMMA LOCI; CUTTING EDGE; EXPRESSION; EFFECTOR; ACETYLATION; PROTEIN; DIFFERENTIATION; EOMESODERMIN; RECRUITMENT; HOMEOSTASIS;

D O I：

10.1016/j.immuni.2009.05.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Memory lymphocytes are characterized by their ability to exhibit a rapid response to the recall antigen, in which differential transcription is important, yet the underlying mechanism is not understood. We report here a genome-wide analysis of histone methylation on two histone H3 lysine residues (H3K4me3 and H3K27me3) and gene expression profiles in naive and memory CD8(+) T cells. We found that specific correlation exists between gene expression and the amounts of H3K4me3 (positive correlation) and H3K27me3 (negative correlation) across the gene body. These correlations displayed four distinct modes (repressive, active, poised, and bivalent), reflecting different functions of these genes in CD8(+) T cells. Furthermore, a permissive chromatin state of each gene was established by a combination of different histone modifications. Our findings reveal a complex regulation by histone methylation in differential gene expression and suggest that histone methylation may be responsible for memory CD8(+) T cell function.

引用

页码：912 / 925

页数：14

共 50 条

[1] The role of models in understanding CD8+ T-cell memory [J].