Separation of catechins from green tea using carbon dioxide extraction

被引：108

作者：

Chang, CMJ ^{[1
]}

Chiu, KL

Chen, YL

Chang, CY

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 40227, Taiwan

[2] Taiwan Tea Expt Stn, Pushin 32613, Taiwan

来源：

FOOD CHEMISTRY | 2000年 / 68卷 / 01期

关键词：

carbon dioxide; catechins; caffeine; polyphenols; co-solvent;

D O I：

10.1016/S0308-8146(99)00176-4

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study presents a novel packed-column extractor coupled with an absorption system to improve the quality of green tea essential oils, extracted by using high pressure carbon dioxide. The effects of various co-solvents on the extract are also examined. In addition, gravimetric measurement and HPLC chromatographic analyses individually determine the amount of essential oil and the concentration of four major catechins. Results show that the mean contents in the extract are 4.4-fold higher by addition of 95% ethanol than by addition of water. The ratio of polyphenols to caffeine is highest in the Soxhlet ethanol extraction. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：109 / 113

页数：5

共 16 条

[1] Method for determining the content of catechins in tea infusions by high-performance liquid chromatography
Bronner, WE
Beecher, GR
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 805 (1-2) : 137 - 142
[2] Comparison of (+)-catechin determination in human plasma by high-performance liquid chromatography with two types of detection: fluorescence and ultraviolet
Carando, S
Teissedre, PL
Cabanis, JC
[J]. JOURNAL OF CHROMATOGRAPHY B, 1998, 707 (1-2): : 195 - 201
[3] Green tea epigallocatechin gallate shows a pronounced growth inhibitory effect on cancerous cells but not on their normal counterparts
Chen, ZP
Schell, JB
Ho, CT
Chen, KY
[J]. CANCER LETTERS, 1998, 129 (02) : 173 - 179
[4] Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography
Dalluge, JJ
Nelson, BC
Thomas, JB
Sander, LC
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 793 (02) : 265 - 274
[5] A polyphenolic pigment from black tea
Davis, AL
Lewis, JR
Cai, Y
Powell, C
Davis, AP
Wilkins, JPG
Pudney, P
Clifford, MN
[J]. PHYTOCHEMISTRY, 1997, 46 (08) : 1397 - 1402
[6] Simultaneous analysis of individual catechins and caffeine in green tea
Goto, T
Yoshida, Y
Kiso, M
Nagashima, H
[J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 749 (1-2) : 295 - 299
[7] Lack E, 1993, EXTRACTION NATURAL P, P101
[8] Green tea polyphenols inhibit oxidant-induced DNA strand breakage in cultured lung cells
Leanderson, P
Faresjo, AO
Tagesson, C
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 1997, 23 (02) : 235 - 242
[9] Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells
Lin, YL
Juan, IM
Chen, YL
Liang, YC
Lin, JK
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (06) : 1387 - 1394
[10] Growth inhibition of leukemic-cells by (-)-epigallocatechin gallate, the main constituent of green tea
Otsuka, T
Ogo, T
Eto, T
Asano, Y
Suganuma, M
Niho, Y
[J]. LIFE SCIENCES, 1998, 63 (16) : 1397 - 1403

← 1 2 →