Creation of cross-linked electrospun isotypic-elastin fibers controlled cell-differentiation with new cross-linker

被引：32

作者：

Miyamoto, Keiichi ^{[1
]}

Atarashi, Masaki ^{[1
]}

Kadozono, Hideki ^{[1
]}

Shibata, Masakazu ^{[1
]}

Koyama, Yoshihiro ^{[1
]}

Okai, Masanori ^{[1
]}

Inakuma, Akinobu ^{[1
]}

Kitazono, Eiichi ^{[2
]}

Kaneko, Hiroaki ^{[2
]}

Takebayashi, Takafumi ^{[1
]}

Horiuchi, Takashi ^{[1
]}

机构：

[1] Mie Univ, Grad Sch Engn, Div Chem Mat, Tsu, Mie 5148507, Japan

[2] Teijin Ltd, TEIJIN Technol Innovat Ctr, Tokyo 1918512, Japan

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2009年 / 45卷 / 01期

关键词：

Tissue engineering; Elastin; Vascular smooth muscle cells; Differentiation; Electrospinning; HUMAN TROPOELASTIN; COLLAGEN; RECEPTOR; ATHEROSCLEROSIS; COACERVATION; BIOMATERIAL; MATRICES; TISSUES;

D O I：

10.1016/j.ijbiomac.2009.03.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effective application of elastin materials for vascular grafts in tissue engineering requires these materials to retain the elastic and biological properties of native elastin. To clarify the influence of soluble elastin isotypes on vascular smooth muscle cells (VSMCs), soluble elastin was prepared from insoluble elastin by hydrolysis with oxalic acid. Its fractions were separated and classified into three isotypes. Elastin retaining 2.25 mol% of cross-linked structures exhibited significant differentiation of VSMCs, which adhered to the elastin with contraction phenotypes similar to that of native elastin, causing proliferation to cease. This trend was more strongly demonstrated in cotton-like elastin fibers with a new cross-linker. The results suggest that elastin isotypes could be applied as new effective biomaterials for suppressing intimal hyperplasia in vascular grafts. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：33 / 41

页数：9

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