Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an Indicator

被引：56

作者：

Ding, Jiawang ^{[1
,2
,3
]}

Lei, Jiahong ^{[1
,2
,3
]}

Ma, Xia ^{[1
,2
]}

Gong, Jun ^{[1
,2
]}

Qin, Wei ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China

[2] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 19期

基金：

中国国家自然科学基金;

关键词：

IN-VITRO SELECTION; SENSITIVE DETECTION; SALMONELLA-TYPHIMURIUM; APTAMER; BACTERIA; BIOSENSOR; IDENTIFICATION;

D O I：

10.1021/ac502335g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Exposure to pathogens in recreational or drinking water is a serious public health concern. It is important to rapidly determine and identify trace levels of pathogens in real environmental samples. We report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of Listeria monocytogenes (LM), a pathogen widely distributed in the environment. An aptamer binds specifically to internalin A, a surface protein present in LM cells. The target-binding event prevents the aptamer from electrostatically interacting with protamine, which can be sensitively detected using a polycation-sensitive membrane electrode. Using this method, LM can be detected down to 10 CFU mL(-1). Coupled to an online filtration system, the bioassay has been evaluated with spiked coastal seawater samples and shows good recovery and high accuracy. This work demonstrates the possibility of developing potentiometric aptasensors for determination and identification of various bacteria in environmental samples.

引用

页码：9412 / 9416

页数：5

共 35 条

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