Structural Studies of an RNA Aptamer Containing GGA Repeats Under Ionic Conditions Using Microchip Electrophoresis, Circular Dichroism, and 1D-NMR

被引：18

作者：

Nishikawa, Fumiko ^{[1
]}

Murakami, Kazuyoshi ^{[1
,2
]}

Matsugami, Akimasa ^{[3
]}

Katahira, Masato ^{[3
,4
]}

Nishikawa, Satoshi ^{[1
,2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Age Dimens Res Ctr, Tsukuba, Ibaraki 3058566, Japan

[2] Univ Tsukuba, Grad Sch Life & Environm Sci, Ibaraki, Japan

[3] Yokohama City Univ, Int Grad Sch Arts & Sci, Yokohama, Kanagawa 232, Japan

[4] JST, PRESTO, Tokyo, Japan

来源：

OLIGONUCLEOTIDES | 2009年 / 19卷 / 02期

关键词：

TELOMERIC DNA; G-QUADRUPLEX; APICAL LOOP; PROMOTER; GENE; SEQUENCE; HAIRPIN; TETRADS; BINDING; SYSTEMS;

D O I：

10.1089/oli.2008.0167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear magnetic resonance (NMR) studies have shown that RNA/DNA oligomers with GGA repeat sequences contain unique G-quadruplex structures in the presence of K+ or Na+ ions. In this study, we used microchip electrophoresis to study the structure of an RNA aptamer against bovine prion protein that possessed four GGA-triplet repeats (wt2). We analyzed the structural changes and characterized dimer formation of the aptamer. Mutational, circular dichroism, and one-dimensional NMR studies of wt2 revealed that K+ ions induce wt2 to assume a thermostable dimer in an intramolecular G-quadruplex with parallel orientation.

引用

页码：179 / 189

页数：11

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