The application of quantum dots, gold nanoparticles and molecular switches to optical nucleic-acid diagnostics

被引:128
作者
Algar, W. Russ [1 ]
Massey, Melissa [1 ]
Krull, Ulrich J. [1 ]
机构
[1] Univ Toronto, Dept Chem & Phys Sci, Chem Sensors Grp, Mississauga, ON L5L 1C6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Colorimetry; Electrochemiluminescence; Fluorescence; Fluorescence resonance energy transfer; Gold nanoparticle; Molecular beacon; Molecular switch; Nucleic acid; Quantum dot; Silver nanoparticle; IN-SITU HYBRIDIZATION; SINGLE-NUCLEOTIDE POLYMORPHISM; RESONANCE ENERGY-TRANSFER; LIGHT-UP PROBES; THIAZOLE ORANGE; DNA HYBRIDIZATION; LABELED DNA; SEMICONDUCTOR NANOCRYSTALS; CHEMILUMINESCENT DETECTION; FLUORESCENCE SPECTROSCOPY;
D O I
10.1016/j.trac.2008.11.012
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This review discusses the application of quantum dots (QDs), gold nanoparticles (NPs), and molecular switches in optical nucleic-acid diagnostics. The size-dependent optical properties of nanoscale materials, as well as the ability to tailor both material and surface composition of NPs, create exciting new possibilities in nucleic-acid analyses. Similarly, molecular assemblies of nucleic acids that generate "on-off" responses at the single-molecule (or particle) level offer significant advantages in diagnostics. Areas of interest in this review include: 1) QDs and fluorescence-in-situ-hybridization, microarrays, fluorescence resonance energy transfer and signal enhancement; 2) gold or silver NPs and colorimetric detection, fluorescence quenching, chemiluminescence, and electrochemiluminescence; and, 3) advances in the design of molecular beacons, hairpin probes and other diagnostic nucleic-acid constructs. We provide brief perspectives on the potential application of lanthanide NPs as optical probes, and the potential of nucleic-acid diagnostics in living cells. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:292 / 306
页数:15
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