The application of quantum dots, gold nanoparticles and molecular switches to optical nucleic-acid diagnostics

被引：128

作者：

Algar, W. Russ ^{[1
]}

Massey, Melissa ^{[1
]}

Krull, Ulrich J. ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem & Phys Sci, Chem Sensors Grp, Mississauga, ON L5L 1C6, Canada

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2009年 / 28卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Colorimetry; Electrochemiluminescence; Fluorescence; Fluorescence resonance energy transfer; Gold nanoparticle; Molecular beacon; Molecular switch; Nucleic acid; Quantum dot; Silver nanoparticle; IN-SITU HYBRIDIZATION; SINGLE-NUCLEOTIDE POLYMORPHISM; RESONANCE ENERGY-TRANSFER; LIGHT-UP PROBES; THIAZOLE ORANGE; DNA HYBRIDIZATION; LABELED DNA; SEMICONDUCTOR NANOCRYSTALS; CHEMILUMINESCENT DETECTION; FLUORESCENCE SPECTROSCOPY;

D O I：

10.1016/j.trac.2008.11.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review discusses the application of quantum dots (QDs), gold nanoparticles (NPs), and molecular switches in optical nucleic-acid diagnostics. The size-dependent optical properties of nanoscale materials, as well as the ability to tailor both material and surface composition of NPs, create exciting new possibilities in nucleic-acid analyses. Similarly, molecular assemblies of nucleic acids that generate "on-off" responses at the single-molecule (or particle) level offer significant advantages in diagnostics. Areas of interest in this review include: 1) QDs and fluorescence-in-situ-hybridization, microarrays, fluorescence resonance energy transfer and signal enhancement; 2) gold or silver NPs and colorimetric detection, fluorescence quenching, chemiluminescence, and electrochemiluminescence; and, 3) advances in the design of molecular beacons, hairpin probes and other diagnostic nucleic-acid constructs. We provide brief perspectives on the potential application of lanthanide NPs as optical probes, and the potential of nucleic-acid diagnostics in living cells. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：292 / 306

页数：15

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