Merging colloidal nanoplasmonics and surface plasmon resonance spectroscopy for enhanced profiling of multiple myeloma-derived exosomes

被引：72

作者：

Di Noto, Giuseppe ^{[1
,2
]}

Bugatti, Antonella ^{[1
,2
]}

Zendrini, Andrea ^{[1
,2
]}

Mazzoldi, Elena Laura ^{[1
,2
]}

Montanelli, Alessandro ^{[3
]}

Caimi, Luigi ^{[1
,2
]}

Rusnati, Marco ^{[1
,2
]}

Ricotta, Doris ^{[1
,2
]}

Bergese, Paolo ^{[1
,2
]}

机构：

[1] Univ Brescia, Dept Mol & Translat Med, I-25132 Brescia, Italy

[2] Univ Brescia, INSTM, I-25132 Brescia, Italy

[3] Spedali Civil Brescia, Clin Chem Lab, I-25123 Brescia, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 77卷

关键词：

Multiple myeloma; Exosome; Nanoplasmonics; Surface plasmon resonance spectroscopy; NANOPARTICLES; MEMBRANES; VESICLES; FUTURE; SIZE;

D O I：

10.1016/j.bios.2015.09.061

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

A novel approach for sorting exosomes from multiple myeloma (MM), monoclonal gammopathy of undetermined significance (MGUS) and healthy individuals is presented. The method is based on the combination of colloidal gold nanoplasmonics and surface plasmon resonance (SPR) biosensing and probes distinctive colloidal properties of MM-derived exosomes, such as molar concentration and cell membrane binding preferences. It allowed to discover that MM patients produce about four folds more exosomes than MGUS and healthy individuals. In addition, it showed that among the analyzed exosomes, only the MM-derived ones bind heparin - a structural analog of heparan sulfate proteoglycans known to mediate exosome endocytosis - with an apparent dissociation constant (K-d) equal to about 1 nM, indicating a high affinity binding. This plasmonic method complements the classical biochemical profiling approach to exosomes, expanding the MM biomarker panel and adding biosensors to the toolbox to diagnose MM. It may find applications for other diseases and has wider interest for fundamental and translational research involving exosomes. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：518 / 524

页数：7

共 37 条

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Exosome Encased Spherical Nucleic Acid Gold Nanoparticle Conjugates as Potent MicroRNA Regulation Agents [J].