Nitric oxide-mediated bystander effect induced by heavy-ions in human salivary gland tumour cells

被引:142
作者
Shao, C
Furusawa, Y
Aoki, M
Matsumoto, H
Ando, K
机构
[1] Natl Inst Radiol Sci, Heavy Ion Radiobiol Res Grp, Inage Ku, Chiba 2638555, Japan
[2] Fukui Med Univ, Dept Expt Radiol, Fukui 9101193, Japan
[3] Fukui Med Univ, Dept Hlth Phys, Fukui 9101193, Japan
关键词
D O I
10.1080/09553000210149786
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Purpose. To investigate the signal factor and its function in the medium-mediated bystander effect during heavy-ion irradiation of human salivary gland (HSG) neoplastic cells. Materials and methods: Unirradiated recipient HSG cells were co-cultivated with HSG donor cells irradiated with 290 MeV/u carbon beams having different LET values. Cell proliferation and nucronucleus (MN) induction in recipient cells with and without treatment of a NO scavenger (PTIO) were measured and the concentration of nitrite in the co-culture medium was detected. As a direct control, the effects of a nitric oxide (NO) generator (sper/NO) on cell proliferation and MN induction were also examined. Results: Increases in cell proliferation and MN induction were found in the recipient HSG cells as a result of co-culturing and cell proliferation was obviously enhanced during a further subculture. In comparison with 13 keV/mum, 100 keV/mum carbon-ion irradiation was found to be a more efficient inducer of the medium-mediated bystander effect. The treatment of cells by PTIO resulted in elimination of such effects, which supports a role for NO in the medium-mediated bystander effect. As an oxidization product of NO, nitrite was detected in the co-culture medium, and the dose-response for its concentration was similar to that of cell proliferation and MN induction in the recipient cells. When the HSG cells were treated by sper/NO with a concentration of less than 20 muM, cell proliferation was enhanced, whereas MN increased along with sper/NO concentration. Conclusion: NO participated in the medium-mediated bystander effects on cell proliferation and MN induction, depending on the LET of irradiation.
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页码:837 / 844
页数:8
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