Dereplication and de novo sequencing of nonribosomal peptides

被引：71

作者：

Ng, Julio ^{[2
]}

Bandeira, Nuno ^{[1
]}

Liu, Wei-Ting ^{[3
]}

Ghassemian, Majid ^{[3
]}

Simmons, Thomas L. ^{[4
]}

Gerwick, William H. ^{[4
,5
]}

Linington, Roger ^{[6
]}

Dorrestein, Pieter C. ^{[3
,5
]}

Pevzner, Pavel A. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Bioinformat Program, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[6] Univ Calif Santa Cruz, Dept Chem, Santa Cruz, CA 95064 USA

来源：

NATURE METHODS | 2009年 / 6卷 / 08期

基金：

美国国家卫生研究院;

关键词：

TANDEM MASS-SPECTROMETRY; CYCLIC-PEPTIDES; IDENTIFICATION;

D O I：

10.1038/nmeth.1350

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but nonidentical NRPs, substantially reducing dereplication efforts.

引用

页码：596 / U65

页数：5

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