Applications of CpG island microarrays for high-throughput analysis of DNA methylation

被引：58

作者：

Yan, PS

Chen, CM

Shi, HD

Rahmatpanah, F

Wei, SH

Huang, TMH ^{[1
]}

机构：

[1] Univ Missouri, Dept Pathol & Anat Sci, Ellis Fischel Canc Ctr, Columbia, MO 65203 USA

[2] Natl Chung Hsing Univ, Dept Zool, Life Sci Coll, Taichung 402, Taiwan

来源：

JOURNAL OF NUTRITION | 2002年 / 132卷 / 08期

关键词：

DNA methylation; CpG islands; microarray;

D O I：

10.1093/jn/132.8.2430S

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.

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收藏

页码：2430S / 2434S

页数：5

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