RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40

被引：68

作者：

Simpson, Laura J. ^{[1
]}

Ross, Anna-Laura ^{[1
]}

Szuts, David ^{[1
]}

Alviani, Cherry A. ^{[1
]}

Oestergaard, Vibe H. ^{[1
]}

Patel, Ketan J. ^{[1
]}

Sale, Julian E. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Div Prot & Nucle Acid Chem, Cambridge CB2 2QH, England

来源：

EMBO REPORTS | 2006年 / 7卷 / 09期

基金：

英国医学研究理事会;

关键词：

DNA damage tolerance; PCNA; RAD18; translesion synthesis; ubiquitination;

D O I：

10.1038/sj.embor.7400777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitination of proliferating- cell nuclear antigen ( PCNA) at K164 by RAD6/ RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell. As in yeast, mutation of K164 of PCNA to arginine in the avian cell line DT40 results in sensitivity to DNA damage but, by contrast, the DT40 pcnaK164R mutant is more sensitive than the rad18 mutant. Consistent with this, we show the presence of residual ubiquitination of PCNA at K164 in the absence of functional RAD18, suggesting the presence of an alternate PCNA ubiquitinating enzyme in DT40. Furthermore, RAD18 and PCNA K164 have non- overlapping roles in the suppression of sister chromatid exchange in DT40, showing that RAD18 has other functions that do not involve the ubiquitination of PCNA.

引用

页码：927 / 932

页数：6

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