A molecular approach for early monitoring of decay basidiomycetes in wood chips

被引：28

作者：

Adair, S ^{[1
]}

Kim, SH ^{[1
]}

Breuil, C ^{[1
]}

机构：

[1] Univ British Columbia, Dept Wood Sci, Chair Forest Prod Biotechnol, Vancouver, BC V6T 1Z4, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 211卷 / 01期

关键词：

decay basidiomycete; wood chips; restriction fragment length polymorphism; rDNA;

D O I：

10.1111/j.1574-6968.2002.tb11212.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA-based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood-decaying basidiomycetes and 20 species of wood-inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR using the ITS1-F-NL2 rDNA primer pair and analysed through sequencing and restriction digestion. A decay basidiomycete-specific restriction fragment length polyrnorphism (RFLP) pattern was generated by DraI. Using this PCR-RFLP method the detection of decay fungi is possible 4 days after the inoculation of the wood chips with either a single culture or mixed species. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：117 / 122

页数：6

共 17 条

[1]

Bridge PD, 1998, APPLICATIONS OF PCR IN MYCOLOGY, P63

[2] Immunological detection of wood decay fungi - An overview of techniques developed from 1986 to the present [J].

Clausen, CA .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 1997, 39 (2-3) :133-143

[3]

CLUBBE CP, 1978, IRGWP186

[4]

COWLING EB, 1961, USDA TECH B 1, V258, P1

[5] A molecular approach for the diagnosis of wood rotting disease in desert citrus [J].

Demetriou, MC ;

Thompson, GA ;

Wright, GC ;

Taylor, KC .

MYCOLOGIA, 2000, 92 (06) :1214-1219

[6]

Edel V, 1998, APPLICATIONS OF PCR IN MYCOLOGY, P1

[7] ITS PRIMERS WITH ENHANCED SPECIFICITY FOR BASIDIOMYCETES - APPLICATION TO THE IDENTIFICATION OF MYCORRHIZAE AND RUSTS [J].

GARDES, M ;

BRUNS, TD .

MOLECULAR ECOLOGY, 1993, 2 (02) :113-118

[8]

HIGHLEY TL, 1987, MATER ORGANISMEN, V22, P39

[9] Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA [J].

Jasalavich, CA ;

Ostrofsky, A ;

Jellison, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (11) :4725-4734

[10]

Kim SH, 1999, APPL ENVIRON MICROB, V65, P287

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