Lifetime-based sensing: Influence of the microenvironment

被引:43
作者
Draxler, S [1 ]
Lippitsch, ME [1 ]
机构
[1] KARL FRANZENS UNIV GRAZ,INST PHYS EXPTL,A-8010 GRAZ,AUSTRIA
关键词
D O I
10.1021/ac9507092
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The influence of the microenvironment on the fluorescence behavior of indicator molecules is investigated. A model is developed to describe the fluorescence decay of indicator molecules in a nonuniform medium. Its consequences for fluorescence lifetime-based chemical sensors are discussed and verified in two examples, namely, a pH sensor using a pyrene compound in a hydrogel and a ruthenium complex for oxygen sensing embedded in a polystyrene membrane.
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页码:753 / 757
页数:5
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