Phosphorothiolate ribozyme against the conserved sequence in V3 loop of HIV-1

被引:8
作者
Zhang, XY
Yamada, R
Shimayama, T
Imada, K
Uchiyama, T
Hattori, T
机构
[1] KYOTO UNIV,INST VIRUS RES,LAB AIDS IMMUNOL,SAKYO KU,KYOTO 606,JAPAN
[2] HITACHI CHEM CO LTD,TOKYO,JAPAN
关键词
D O I
10.1006/bbrc.1996.1827
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescent-labeled phosphorothiolate DNA-RNA chimeric hammerhead ribozyme (thio-Rz) was synthesized. The ribozyme recognizes relatively conserved sequences in the functionally important region (V3 loop) of envelope protein of HIV-1. The thio-Rz as well as wild-type ribozyme cleaved RNA transcripts (414 base) of V3 region in a dose-dependent manner. After delivery into the cells, both wild-type and thio-Rz were able to be readily detected. The positive signals faded within 12 h in the former case, while they lasted 24 h in the latter case. The inhibitory effect by thio-Rz on cell fusion between envelope-expressing CHO cells and CD4(+) cells appeared to be time-dependent, and about 70% of inhibition was seen at 72 h. Possible applications of the ribozyme against HIV-1 infection were discussed. (C) 1996 Academic Press, Inc.
引用
收藏
页码:466 / 471
页数:6
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