Quantitative analysis of cannabinoids from Cannabis sativa using 1H-NMR

被引:70
作者
Hazekamp, A [1 ]
Choi, YH [1 ]
Verpoorte, R [1 ]
机构
[1] Leiden Univ, Div Pharmacognosy, Inst Biol, NL-2300 RA Leiden, Netherlands
关键词
cannabinoid; H-1-NMR spectroscopy; quantitative analysis; Cannabis sativa;
D O I
10.1248/cpb.52.718
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A H-1-NMR method has been developed for the quantitative analysis of pure cannabinoids and for cannabinoids present in Cannabis sativa plant material without any chromatographic purification. The experiment was performed by the analysis of singlets in the range of delta 4.0-7.0 in the H-1-NMR spectrum, in which distinguishable signals of each cannabinoid are shown. Quantitation was performed by calculating the relative ratio of the peak area of selected proton signals of the target compounds to the known amount of the internal standard, anthracene. For this method no reference compounds are needed. It allows rapid and simple quantitation of cannabinoids with a final analysis time of only 5 min without the need for a pre-purification step.
引用
收藏
页码:718 / 721
页数:4
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