Targeted gene correction: Synthesis and characterization of double-hairpin 2'-O-methyl RNA/DNA chimera oligonucleotides

被引：2

作者：

Andrus, A

Vinayak, R

Kuimelis, RG

Mullah, B

机构：

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期

关键词：

DNA OLIGONUCLEOTIDE; RNA; NUCLEOSIDES; CHEMISTRY; SUPPORT;

D O I：

10.1080/07328319708006155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of 2'-O-methyl RNA/DNA chimera oligonucleotides were synthesized with a double-hairpin structural motif. Liposome formulated delivery of the chimeras effected targeted, high conversion of mutant alleles in mammalian cell culture. The chimera oligonucleotides were prepared with DNA and 2'-OMe RNA phosphoramidite nucleoside monomers on the ABI 394 synthesizer.

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页码：1183 / 1194

页数：12

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