Structural analysis of the extracellular polysaccharide produced by Sphaerotilus natans

被引：16

作者：

Takeda, M ^{[1
]}

Nomoto, S ^{[1
]}

Koizumi, J ^{[1
]}

机构：

[1] Yokohama Natl Univ, Div Sci Mat & Chem Engn, Fac Engn, Kanagawa 2408501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 07期

关键词：

Sphaerotilus natans; extracellular polysaccharide; structural analysis;

D O I：

10.1271/bbb.66.1546

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An extracellular polysaccharide (EPS) was recovered and purified from the culture fluid of a sheathed bacterium, Sphaerotilus natans. Glucose, rhamnose, and aldobiouronic acid were detected in the acid hydrolysate of EPS by thin-layer chromatography (TLC). The aldobiouronic acid was found to be composed of glucuronic acid and rhamnose by TLC and gas-liquid chromatography analyses of the corresponding neutral disaccharide. The structure of EPS was identified by methylation linkage analysis and nuclear magnetic resonance. Additionally, partial acid hydrolysates of EPS were prepared and put through fast atom bombardment-mass spectrometry to determine the sugar sequence of EPS. The resulting data showed that EPS produced by S. natans is a new gellan-like polysaccharide constructed from a tetrasaccharide repeating unit, as shown below. --> 4)-alpha-n-Glc p-(1 --> 2)-beta-D-GlcA p-(1 --> 2)-alpha-L-Rha p- (1 --> 3)-beta-L-Rha p-(1 -->.

引用

页码：1546 / 1551

页数：6

共 26 条

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