Genotoxic effects of cadmium chloride and azadirachtin treated singly and in combination in fish

The genotoxic effects of cadmium chloride (CdCl2) and azadirachtin (Aza) were assessed singly and conjointly in a fish, Oreochromis mossambicus, with endpoints such as chromosome aberrations, abnormal red cell nuclei, abnormal sperm morphology, and protein content (both qualitative and quantitative) of selected tissues, namely, muscle, heart, eye, brain, gill, liver, spleen, and kidney. The primary objectives were, first, to examine if CdCl2, a common pollutant, and AM, a natural product of the neem plant used extensively as an 'ecofriendly' agent for many purposes, had any genotoxic, effect of their own on nontarget aquatic organisms of economic importance; and second, if Aza could have any ameliorating effect on CdCl2-induced genotoxicity in O. mossambicus tissues. As compared with distilled water-treated controls, both CdCl2 and Aza induced genotoxicity in O. mossambicus, the former in greater quantity than that produced by Aza. However, Cd-induced toxicity in O. mossambicus appeared to be ameliorated to some extent by Aza. (C) 2004 Elsevier Inc. All rights reserved.