A high capacity in vitro assay for measuring the cytoadherence of Plasmodium falciparum-infected erythrocytes

被引:20
作者
Prudhomme, JG [1 ]
Sherman, IW [1 ]
机构
[1] Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA
关键词
lactic dehydrogenase; Plasmodium falciparum; pLDH; malaria; cytoadherence; microassay;
D O I
10.1016/S0022-1759(99)00098-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, efficient, sensitive, reproducible and high throughput assay for measuring the cytoadhesion of Plasmodium falciparum-infected red blood cells (human malaria) is described. The assay format uses 96-well microplates, with the number of P. falciparum parasitized erythrocytes bound determined by measuring Plasmodium specific lactic dehydrogenase activity colorimetrically (absorbance at 655 nm) using the 3-acetylpyridine analog of nicotinamide adenine dinucleotide, nitro blue tetrazolium and diaphorase. The results of the described microplate assay were found to be comparable to those using microscopic analysis but much less time consuming. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:169 / 176
页数:8
相关论文
共 21 条
[1]   ADHESION OF PARASITIZED RED-BLOOD-CELLS TO CULTURED ENDOTHELIAL-CELLS - A FLOW-BASED STUDY OF ISOLATES FROM GAMBIAN CHILDREN WITH FALCIPARUM-MALARIA [J].
COOKE, BM ;
MORRISJONES, S ;
GREENWOOD, BM ;
NASH, GB .
PARASITOLOGY, 1993, 107 :359-368
[2]  
CRANDALL I, 1994, METHOD CELL BIOL, V45, P193
[3]   Inhibitory activity of human lactoferrin and its peptide on chondroitin sulfate A-, CD36-, and thrombospondin-mediated cytoadherence of Plasmodium falciparum-infected erythrocytes [J].
Eda, S ;
Eda, K ;
Prudhomme, JG ;
Sherman, IW .
BLOOD, 1999, 94 (01) :326-332
[4]  
EVANS CW, 1991, MEASURING CELL ADHES, P113
[5]   AN IMPROVED MICROASSAY FOR PLASMODIUM-FALCIPARUM CYTOADHERENCE USING STABLE TRANSFORMANTS OF CHINESE-HAMSTER OVARY CELLS EXPRESSING CD36 OR INTERCELLULAR-ADHESION MOLECULE-1 [J].
HASLER, T ;
ALBRECHT, GR ;
VANSCHRAVENDIJK, MR ;
AGUIAR, JC ;
MOREHEAD, KE ;
PASLOSKE, BL ;
MA, C ;
BARNWELL, JW ;
GREENWOOD, B ;
HOWARD, RJ .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 48 (03) :332-347
[6]   SYNCHRONIZATION OF PLASMODIUM-FALCIPARUM ERYTHROCYTIC STAGES IN CULTURE [J].
LAMBROS, C ;
VANDERBERG, JP .
JOURNAL OF PARASITOLOGY, 1979, 65 (03) :418-420
[7]   Plasmodium falciparum:: Thrombospondin mediates parasitized erythrocyte band 3-related adhesin binding [J].
Lucas, JZ ;
Sherman, IW .
EXPERIMENTAL PARASITOLOGY, 1998, 89 (01) :78-85
[8]   MEASUREMENT OF THE LACTATE-DEHYDROGENASE ACTIVITY OF PLASMODIUM-FALCIPARUM AS AN ASSESSMENT OF PARASITEMIA [J].
MAKLER, MT ;
HINRICHS, DJ .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 48 (02) :205-210
[9]   INTER-CELLULAR RECOGNITION - QUANTITATION OF INITIAL BINDING EVENTS [J].
MCCLAY, DR ;
WESSEL, GM ;
MARCHASE, RB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08) :4975-4979
[10]   SEPARATION OF VIABLE SCHIZONT-INFECTED RED-CELLS OF PLASMODIUM-FALCIPARUM FROM HUMAN-BLOOD [J].
PASVOL, G ;
WILSON, RJM ;
SMALLEY, ME ;
BROWN, J .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1978, 72 (01) :87-88