Repair of articular cartilage defects with cultured chondrocytes in Atelocollagen gel - Comparison with cultured chondrocytes in suspension

被引:99
作者
Katsube, K [1 ]
Ochi, M [1 ]
Uchio, Y [1 ]
Maniwa, S [1 ]
Matsusaki, M [1 ]
Tobita, M [1 ]
Iwasa, J [1 ]
机构
[1] Shimane Med Univ, Dept Orthopaed, Izumo, Shimane 6938501, Japan
关键词
D O I
10.1007/PL00021232
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
We attempted to repair full-thickness articular cartilage defects in rabbit knee joints with allogeneic cultured chondrocytes embedded in Atelocollagen gel. An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with chondrocytes cultured in the collagen gel and covered with periosteal flap (G group). In three other experimental groups, the same defects were transplanted with chondrocytes in monolayer culture with periosteal flap (M group), periosteal graft only (P group), or left empty (E group). At 4, 12, and 24 weeks after operation, the reparative tissue was analyzed macroscopically and histologically. At 4 weeks after operation, the surfaces of the reparative tissue were smooth, and the defects were filled with reparative tissues that resembled hyaline cartilage in all four groups. However, the reparative tissues degenerated gradually with time in the M, P, and E groups. In contrast, in the G group, the reparative tissue retained its thickness, and there was a steady integration of the grafted tissue into the adjacent normal cartilage at 24 weeks after operation. The results suggest that transplantation of allogeneic chondrocytes cultured in Atelocollagen gel is effective in repairing an articular cartilage defect.
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页码:121 / 127
页数:7
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