Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1.

被引：42

作者：

Graham, KA

Leithoff, J

Coe, IR

Mowles, D

Mackey, JR

Young, JD

Cass, CE ^{[1
]}

机构：

[1] Univ Alberta, Cross Canc Inst, Dept Oncol, Edmonton, AB T6G 1Z2, Canada

[2] York Univ, Dept Biol, N York, ON M3J 1P3, Canada

[3] Univ Alberta, Dept Physiol, Edmonton, AB T6G 2H7, Canada

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2000年 / 19卷 / 1-2期

关键词：

D O I：

10.1080/15257770008033018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transportability of cytosine-containing nucleosides by recombinant hCNT1 was investigated in transfected mammalian cells. Apparent K-m values for hCNT1-mediated transport of uridine, cytidine and deoxycytidine were, respectively, 59, 140 and 150 mu M. Uridine transport was inhibited 89, 32 and 11%, respectively, by 500 CIM gemcitabine, cytarabine and lamivudine, demonstrating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarabine and lamivudine are poor hCNT1 permeants.

引用

页码：415 / 434

页数：20

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