Selected plant species from the Cree pharmacopoeia of northern Quebec possess anti-diabetic potential

被引:79
作者
Spoor, Danielle C. A.
Martineau, Louis C.
Leduc, Charles
Benhaddou-Andaloussi, Ali
Meddah, Bouchra
Harris, Cory
Burt, Andrew
Fraser, Marie-Helene
Coonishish, Jason
Joly, Erik
Cuerrier, Alain
Bennett, Steffany A. L.
Johns, Timothy
Prentki, Marc
Arnason, John T.
Haddad, Pierre S.
机构
[1] Univ Montreal, Dept Pharmacol, Montreal, PQ H3C 3J7, Canada
[2] Univ Laval, Inst Nutraceut & Aliments Fonct, Quebec City, PQ G1K 7P4, Canada
[3] Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
[4] Univ Ottawa, Ctr Res Biopharmaceut & Biotechnol, Ottawa, ON K1N 6N5, Canada
[5] Univ Ottawa, Dept Biochem Microbiol & Immunol, Neural Regenerat Lab, Ottawa, ON K1N 6N5, Canada
[6] McGill Univ, Sch Dietet & Human Nutr, Montreal, PQ H3A 3N6, Canada
[7] Univ Montreal, Dept Nutr, Montreal, PQ H1W 4A4, Canada
[8] CHU Montreal, Ctr Rech, Montreal Diabet Res Ctr, Montreal, PQ H1W 4A4, Canada
[9] Univ Montreal, Montreal Bot Garden, Plant Biol Res Inst, Montreal, PQ H9X 3V9, Canada
关键词
type II diabetes; medicinal plants; cell-based bioassays; insulin action and secretion; insulinomimetic; insulinsensitizing; cytoprotection; anti-oxidant potential; natural health products;
D O I
10.1139/Y06-018
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Type II diabetes is a major health problem worldwide. Some populations, such as aboriginal peoples, are particularly at risk for this disease. In the Cree Nation of Quebec, Canada, prevalence in adults is approaching 20%, and the consequences are compounded by low compliance with modern medicine. In 2003, we conducted an ethnobotanical study of Cree medicinal plants used for the treatment of symptoms of diabetes. This served as the basis for a project designed to identify efficacious complementary treatment options more readily accepted by this population. The present study assesses the in vitro anti-diabetic potential of extracts from the 8 most promising plants to emerge from the ethnobotanical study. Cell-based bioassays were employed to screen for (i) potentiation of glucose uptake by skeletal muscle cells (C2C12) and adipocytes (3T3-L1); (ii) potentiation of glucose-stimulated insulin secretion (GSIS) and insulin production by pancreatic beta cells (INS 832/13); (iii) potentiation of triglyceride accumulation in differentiating 3T3-L1 cells; (iv) protection against glucose toxicity and glucose deprivation in pre-sympathetic neurons (PC12-AC). Additionally, anti-oxidant activity was measured biochemically by the diphenylpicrylhydrazyl (DPPH) reduction assay. All plant extracts potentiated basal or insulin-stimulated glucose uptake to some degree in muscle cells or adipocytes. Adipocyte differentiation was accelerated by 4 extracts. Five extracts conferred protection in PC12 cells. Three extracts displayed free radical scavenging activity similar to known anti-oxidants. None of the plant extracts enhanced GSIS or insulin content in INS 832/13 beta cells. It is concluded that the Cree pharmacopoeia contains several plants with significant anti-diabetic potential.
引用
收藏
页码:847 / 858
页数:12
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