Isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes

被引:40
作者
Sonnenberg, ASM [1 ]
deGroot, PWJ [1 ]
Schaap, PJ [1 ]
Baars, JJP [1 ]
Visser, J [1 ]
vanGriensven, LJLD [1 ]
机构
[1] WAGENINGEN UNIV AGR,SECT MOL GENET IND MICROORGANISMS,NL-6703 HA WAGENINGEN,NETHERLANDS
关键词
D O I
10.1128/AEM.62.12.4542-4547.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The genome of the cultivated basidiomycete Agaricus bisporus Horst U1 and of its homokaryotic parents has been characterized by using an optimized method of pulsed-field gel electrophoresis. Expressed sequence tags obtained as expressed cDNAs from a primordial tissue-derived cDNA library and a number of previously isolated genes were used to identify the individual chromosomes of the parental lines of Horst U1. The genome consists of 13 chromosomes, and its total size is 31 Mb. For those chromosomes that could not be resolved by contour-clamped homogeneous electric field electrophoresis, the segregation of marker genes was studied in a set of 86 homokaryotic offspring of Horst U1. At least two markers were assigned to each individual chromosome. In this way all individual chromosomes were unequivocally identified. The large size difference observed between the homologous chromosomes IX, harboring the rDNA repeat, was shown to be largely due to a higher copy number of rDNA in parental strain H97 than in parental strain H39.
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收藏
页码:4542 / 4547
页数:6
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