The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated Brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity

被引:47
作者
Campbell, S
Gaus, K
Bittman, R
Jessup, W
Crowe, S
Mak, J
机构
[1] Macfarlane Burnet Inst Med Res & Publ Hlth, Melbourne, Vic 3004, Australia
[2] Monash Univ, Dept Med, Sch Med, Melbourne, Vic 3004, Australia
[3] Natl Ctr HIV Virol Res, Melbourne, Vic, Australia
[4] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
[5] Univ New S Wales, Sch Med Sci, Ctr Vasc Res, Randwick, NSW, Australia
[6] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia
[7] CUNY, Dept Chem & Biochem, Queens Coll, Flushing, NY USA
关键词
D O I
10.1128/JVI.78.19.10556-10565.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Lipid rafts are enriched in cholesterol and sphingomyelin and are isolated on the basis of insolubility in detergents, such as Brij 98 and Triton X-100. Recent work by Holm et al. has shown that rafts insoluble in Brig 98 can be found in human immunodeficiency virus type 1 (HIV-1) virus-like particles, although it is not known whether raft-like structures are present in authentic HIV-1 and it is unclear whether a virion-associated raft-like structure is required for HIV replication. Independently, it was previously reported that virion-associated cholesterol is critical for HIV-1 infectivity, although the specific requirement of virion cholesterol in HIV-1 was not examined. In the present study, we have demonstrated that infectious wild-type HIV-1 contains Brij 98 rafts but only minimal amounts of Triton X-100 rafts. To directly assess the functional requirement of virion-associated rafts and various features of cholesterol on HIV-1 replication, we replaced virion cholesterol with exogenous cholesterol analogues that have demonstrated either raft-promoting or -inhibiting capacity in model membranes. We observed that variable concentrations of exogenous analogues are required to replace a defined amount of virion-associated cholesterol, showing that structurally diverse cholesterol analogues have various affinities toward HIV-1. We found that replacement of 50% of virion cholesterol with these exogenous cholesterol analogues did not eliminate the presence of Brij 98 rafts in HIV-1. However, the infectivity levels of the lipid-modified HIV-1s directly correlate with the raft-promoting capacities of these cholesterol analogues. Our data provide the first direct assessment of virion-associated Brij 98 rafts in retroviral replication and illustrate the importance of the raft-promoting property of virion-associated cholesterol in HIV-1 replication.
引用
收藏
页码:10556 / 10565
页数:10
相关论文
共 64 条
[1]   PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].
ADACHI, A ;
GENDELMAN, HE ;
KOENIG, S ;
FOLKS, T ;
WILLEY, R ;
RABSON, A ;
MARTIN, MA .
JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291
[2]   LIPID-COMPOSITION AND FLUIDITY OF THE HUMAN-IMMUNODEFICIENCY-VIRUS ENVELOPE AND HOST-CELL PLASMA-MEMBRANES [J].
ALOIA, RC ;
TIAN, HR ;
JENSEN, FC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) :5181-5185
[3]   Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses [J].
Bavari, S ;
Bosio, CM ;
Wiegand, E ;
Ruthel, G ;
Will, AB ;
Geisbert, TW ;
Hevey, M ;
Schmaljohn, C ;
Schmaljohn, A ;
Aman, MJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 195 (05) :593-602
[4]   HIV-cell fusion - The viral mousetrap [J].
Binley, J ;
Moore, JP .
NATURE, 1997, 387 (6631) :346-348
[5]   Structure and function of sphingolipid- and cholesterol-rich membrane rafts [J].
Brown, DA ;
London, E .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17221-17224
[6]   Functions of lipid rafts in biological membranes [J].
Brown, DA ;
London, E .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 :111-136
[7]   SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].
BROWN, DA ;
ROSE, JK .
CELL, 1992, 68 (03) :533-544
[8]   Virion-associated cholesterol is critical for the maintenance of HIV-1 structure and infectivity [J].
Campbell, SM ;
Crowe, SM ;
Mak, J .
AIDS, 2002, 16 (17) :2253-2261
[9]   Lipid rafts and HIV-1: from viral entry to assembly of progeny virions [J].
Campbell, SM ;
Crowe, SM ;
Mak, J .
JOURNAL OF CLINICAL VIROLOGY, 2001, 22 (03) :217-227
[10]   The structural biology of type I viral membrane fusion [J].
Colman, PM ;
Lawrence, MC .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (04) :309-319