Accurate quantitation of standard peptides used for quantitative proteomics

被引：19

作者：

Bordeerat, Narisa K. ^{[2
]}

Georgieva, Nadia I. ^{[2
]}

Klapper, David G. ^{[3
]}

Collins, Leonard B. ^{[4
]}

Cross, Tyra J. ^{[5
]}

Borchers, Christoph H. ^{[5
,6
]}

Swenberg, James A. ^{[2
,4
]}

Boysen, Gunnar ^{[1
,2
,4
]}

机构：

[1] Univ Arkansas Med Sci, Fay W Boozman Coll Publ Hlth Environm & Occupat H, Dept Environm & Occupat Hlth, Little Rock, AR 72205 USA

[2] Univ N Carolina, Sch Publ Hlth, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Sch Med, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA

[4] Univ N Carolina, Sch Publ Hlth, Ctr Environm Hlth & Susceptibil, Chapel Hill, NC USA

[5] Univ Victoria, Genome British Columbia Prote Ctr, Victoria, BC, Canada

[6] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC, Canada

来源：

PROTEOMICS | 2009年 / 9卷 / 15期

关键词：

Peptide standards; Quality control; Quantitative analysis; AMINO-ACIDS; PROTEIN; QUANTIFICATION; EXPRESSION;

D O I：

10.1002/pmic.200900043

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 mu M. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.

引用

页码：3939 / 3944

页数：6

共 27 条

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