Optimization of extraction technology of Astragalus polysaccharides by response surface methodology and its effect on CD40

被引：52

作者：

Li Rui ^{[1
]}

Chen Wei-chang ^{[1
]}

Wang Wei-peng ^{[2
]}

Tian Wen-yan ^{[1
]}

Zhang Xue-guang ^{[3
]}

机构：

[1] Soochow Univ, Affiliated Hosp 1, Dept Gastroenterol, Key Lab Med & Clin Immunol Jiangsu Prov, Suzhou 215006, Peoples R China

[2] Soochow Univ, Coll Pharm, Suzhou 215007, Peoples R China

[3] Soochow Univ, Inst Med Biotechnol, Suzhou 215007, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2009年 / 78卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Astragalus polysaccharides; CD40; Response surface methodology; Box-Behnken design;

D O I：

10.1016/j.carbpol.2009.06.018

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Astragali is a herbal medicine used as an adjuvant extensively in the treatment of various cancers of lung, digestive tract, urinary system, etc. Statistics-based experimental designs were applied to optimize the extraction conditions for Astragalus polysaccharides (AP). The optimum conditions were found to be: the values of extraction time 3 h, ratio of liquid to solid 4 and particle size 33.8 mesh. Under these optimized conditions, the maximum AP extraction rate was 16.32 (%), which well matches with the predicted value. Furthermore, the chemical composition was analyzed with HPLC, and the pharmacological effect of the AP was evaluated. Results showed that AP could significantly decreased CD40 expression rate and protein expression in rats with stomach cancer. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：784 / 788

页数：5

共 9 条

[1]

Extraction, purification, and characterization of the polysaccharides from Opuntia milpa alta [J].