Cell permeabilization for extraction of penicillin acylase from Escherichia coli by reverse micellar solutions

被引：23

作者：

Bansal-Mutalik, R ^{[1
]}

Gaikar, VG ^{[1
]}

机构：

[1] Univ Bombay, Dept Chem Technol, Bombay 400019, Maharashtra, India

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 32卷 / 01期

关键词：

penicillin acylase; Escherichia coli; reverse micelles; periplasmic enzyme; permeabilization; extraction;

D O I：

10.1016/S0141-0229(02)00178-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Water-in-hexane macro- and micro-emulsions stabilized by sodium bis-(2-ethylhexyl) sulfosuccinate (AOT) were used for selective permeabilization of Escherichia coli cells to extract penicillin acylase (3.5.1.11). Various organic solvents and surfactants were compared for the yield and purification of the enzyme. Recoveries upto 144% with respect to sonication and specific activity of the enzyme upto 26 units mg(-1) have been achieved. Due to low solubilities of AOT and aliphatic hydrocarbons in water the recovered enzyme was free from contaminants. A possible mechanism for cell permeabilization and enzyme purification by reverse micellar treatment is proposed. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：14 / 26

页数：13

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