Direct detection of carcinoembryonic antigen autoantibodies in clinical human serum samples using a surface plasmon resonance sensor

被引：62

作者：

Ladd, Jon ^{[1
]}

Lu, Hailing ^{[2
]}

Taylor, Allen D. ^{[1
]}

Goodell, Vivian ^{[2
]}

Disis, Mary L. ^{[2
]}

Jiang, Shaoyi ^{[1
]}

机构：

[1] Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA

[2] Univ Washington, Div Oncol, Tumor Vaccine Grp, Seattle, WA 98195 USA

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 70卷 / 01期

关键词：

Carcinoembryonic antigen; CEA; Surface plasmon resonance; SPR; Autoantibody; Cancer; Biomarker; SELF-ASSEMBLED MONOLAYERS; PROTEIN IMMOBILIZATION; ANTIBODY ARRAYS; PROSTATE-CANCER; OVARIAN-CANCER; BREAST-CANCER; CELLS; MICROARRAYS; EXPRESSION; BIOSENSORS;

D O I：

10.1016/j.colsurfb.2008.11.032

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The ability to detect biomarkers in human serum is important for cancer diagnostics. The work presented focuses on the establishment of a surface plasmon resonance (SPR) biosensor as a means for detecting varying levels of autoantibody biomarkers in human serum samples. Carcinoembryonic antigen (CEA) is a biomarker that is present in human serum. It is thought that CEA levels become elevated in patients with colon and ovarian cancer, causing a corresponding increase in the autoantibody level in human serum. Detection of this CEA autoantibody increase could be used to diagnose cancer in patients. Using a SPR biosensor, human serum samples were screened directly for CEA antibody levels. Results using a sandwich assay with a SPR sensor demonstrated the same linear trend as seen from an established enzyme-linked immunosorbent assay (ELISA) method. Serum samples from five healthy individuals were used to establish a threshold value for differentiating a cancerous serum sample from a negative sample with a 95% confidence. Three serum samples from cancer patients with positive CEA antibody levels as evaluated by ELISA were used to test the criterion. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1 / 6

页数：6

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