Extraction of azadirachtin A from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

被引：34

作者：

Ambrosino, P

Fresa, R

Fogliano, V

Monti, SM

Ritieni, A

机构：

[1] Univ Naples Federico II, Dipartimento Sci Alimenti, I-80055 Naples, Italy

[2] Univ Salerno, Dipartimento Ingn Chim, I-84084 Salerno, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 12期

关键词：

supercritical fluid extraction; azadirachtin A; HPLC; LC/MS; neem seed kernels;

D O I：

10.1021/jf9905368

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO2) were used as extractive agents in a three-separation-stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.

引用

页码：5252 / 5256

页数：5

共 24 条

[1] X-RAY CRYSTALLOGRAPHIC STRUCTURE DETERMINATION OF DETIGLOYLDIHYDROAZADIRACHTIN AND REASSIGNMENT OF THE STRUCTURE OF THE LIMONOID INSECT ANTIFEEDANT AZADIRACHTIN [J].