Sphingosine 1-phosphate induces arachidonic acid mobilization in A549 human lung adenocarcinoma cells

被引:21
作者
Vasta, V [1 ]
Meacci, E [1 ]
Catarzi, S [1 ]
Donati, C [1 ]
Farnararo, M [1 ]
Bruni, P [1 ]
机构
[1] Univ Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2000年 / 1483卷 / 01期
关键词
sphingosine; 1-phosphate; bradykinin; arachidonic acid; protein kinase C; phospholipase A(2); phospholipase D; A549 epithelial cell;
D O I
10.1016/S1388-1981(99)00183-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the present paper, the effect of sphingosine 1-phosphate (Sph-1-P) on arachidonic acid mobilization in A549 human lung adenocarcinoma cells was investigated. Sph-1-P provoked a rapid and relevant release of arachidonic acid which was similar to that elicited by bradykinin, well-known pro-inflammatory agonist. The Sph-1-P-induced release of arachidonic acid involved Ca2+-independent phospholipase A(2) (iPLA(2)) activity, as suggested by the dose-dependent inhibition exerted by the rather specific inhibitor bromoenol lactone. The Sph-1-P-induced release of arachidonic acid was pertussis toxin-sensitive, pointing at a receptor-mediated mechanism, which involves heterotrimeric Gi proteins. The action of Sph-1-P was totally dependent on protein kinase C (PKC) catalytic activity and seemed to involve agonist-stimulated phospholipase D (PLD) activity. This study represents the first evidence for Sph-1-P-induced release of arachidonic acid which occurs through a specific signaling pathway involving Gi protein-coupled receptor(s), PKC, PLD and iPLA(2) activities. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:154 / 160
页数:7
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