Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis

被引：58

作者：

Guan, YF

Zhang, W ^{[1
]}

Deng, MC

Jin, MF

Yu, XJ

机构：

[1] Chinese Acad Sci, Dalian Inst Chem & Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China

[2] Flinders Univ S Australia, Sch Med, Cooperat Res Ctr, Bedford Pk, SA 5042, Australia

[3] Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 13期

关键词：

carbonylcyanide m-chlorophenylhydrazone (CCCP); green algae; hydrogen; photosynthesis; Platymonas subcordiformis;

D O I：

10.1023/B:BILE.0000032961.71564.00

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A marine green microalga, Platymonas subcordiformis, photo-synthetically generates H-2 but only transiently at a negligible yield when exposed to light after a period of dark anaerobic incubation. A protonophore uncoupler, carbonyl cyanide m-chlorophenylhrazone ( CCCP) significantly increased the yield of H2 photo-production. CCCP optimally at 15 muM gave 4.9 ml H-2 after 8 h light irradiation in 1 l algal cell culture at 1.8 x 10(6) cells ml(-1). The H-2 yield at 15 muM CCCP was increased by 240-fold when compared to the control. This improvement may be by CCCP disrupting the proton motive force thus facilitating proton transfer across the thylakoidal membrane.

引用

页码：1031 / 1035

页数：5

共 12 条

[1] Physiological analyses of the hydrogen gas exchange in cyanobacteria [J].