Simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rat serum for the pharmacokinetic investigations by high performance liquid chromatography-tandem mass spectrometry

被引：31

作者：

Ran, Xiaorong

Liang, Qionglin

Luo, Guoan ^{[1
]}

Liu, Qingfei

Pan, Yanshu

Wang, Bing

Pang, Chunhong

机构：

[1] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

[2] Beijing Univ Tradit Chinese Med, Sch Basic Med Sci, Beijing 100029, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 842卷 / 01期

基金：

中国国家自然科学基金;

关键词：

HPLC-MS-MS; geniposide; baicalin; cholic acid; hyodeoxycholic acid; pharmacokinetics;

D O I：

10.1016/j.jchromb.2006.05.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, and specific analytical method for simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in 50 RL samples of rat serum was developed by high performance liquid chromatography-tandem mass spectrometry. The quantification of the target compounds was determined by multiple reaction monitoring (MRM) mode using electrospray ionization (ESI). The correlation coefficients of the calibration curves were better than 0.997. The intra- and inter-day accuracy, precision, and linear range had been investigated in detail. This method was subsequently applied to pharmacokinetic studies of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rats successfully. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：22 / 27

页数：6

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