Extraction and characterization of native heteroxylans from delignified corn stover and aspen

被引：71

作者：

Naran, Radnaa ^{[1
]}

Black, Stuart ^{[2
]}

Decker, Stephen R. ^{[2
]}

Azadi, Parastoo ^{[1
]}

机构：

[1] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA

[2] Natl Renewable Energy Lab, Chem & Biosci Ctr, Golden, CO 80401 USA

来源：

CELLULOSE | 2009年 / 16卷 / 04期

基金：

美国能源部;

关键词：

Biomass; Biomass delignification; Plant cell wall; Glucuronoxylan; Hardwood xylan; Corn stover xylan; Xylan acetylation; Endoxylanase; Homo- and-heteronuclear NMR-spectroscopy; MALDI-TOF MS; CELL-WALL POLYSACCHARIDES; MASS-SPECTROMETRY; MAIZE COLEOPTILES; BRAN; HEMICELLULOSES; MONOCOTYLEDONS; UNIQUE; XYLAN; ACIDS;

D O I：

10.1007/s10570-009-9324-y

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

Dimethylsulfoxide-solubilized polysaccharides from delignified corn stover and aspen were characterized. The biomass was delignified by two different techniques; a standard acid chlorite and a pulp and paper QPD technique comprising chelation (Q), peroxide (P), and acid-chlorite (D). Major polysaccharides in all fractions were diversely substituted xylan. Xylan acetylation was intact after chlorite delignification and, as expected, xylan from QPD-delignified fraction was de-acetylated by the alkaline peroxide step. The study of DMSO-extractable xylans from chlorite-delignified biomass revealed major differences in native acetylation patterns between corn stover and aspen xylan. Xylan from cell walls of corn stover contains 2-O- and 3-O-mono-acetylated xylan and [MeGlcA-alpha-(1 -> 2)][3-OAc]-xylp units. In addition, aspen xylan also contains 2,3-di-O-acetylated xylose. 1,4-beta-d-xylp residues substituted with MeGlcA at O-2 position are absent in chlorite-delignified aspen xylan. Sugar composition in accord with NMR-spectroscopic data indicated that corn stover xylan is arabinosylated while aspen xylan is not. We have shown that corn stover xylan has similar structure with xylans from other plants of Poales order. No evidence was found to indicate the presence of 1,4-beta-d-[MeGlcA-alpha-(1 -> 2)][Ara-alpha-(1 -> 3)]-xylp in corn stover xylan fractions.

引用

页码：661 / 675

页数：15

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