In Vitro Precursor-Directed Synthesis of Polyketide Analogues with Coenzyme A Regeneration for the Development of Antiangiogenic Agents

被引：30

作者：

Kim, Moon Il ^{[1
]}

Kwon, Seok Joon ^{[1
]}

Dordick, Jonathan S. ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

来源：

ORGANIC LETTERS | 2009年 / 11卷 / 17期

基金：

美国国家卫生研究院;

关键词：

ENDOTHELIAL GROWTH-FACTOR; MALONYL-COA SYNTHETASE; RHIZOBIUM-TRIFOLII; ESCHERICHIA-COLI; CELLS; BIOSYNTHESIS; EXPRESSION; SYNTHASE; INHIBITION; SUPPRESSES;

D O I：

10.1021/ol901243e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Polyketide analogues are produced via in vitro reconstruction of a precursor-directed polyketide biosynthetic pathway. Malonyl-CoA synthetase (MCS) was used in conjunction with chalcone synthase (CHS), thereby allowing efficient use of synthetic starter molecules and malonate as extender. Coenzyme-A was recycled up to 50 times. The use of a simple immobilization procedure resulted in up to a 30-fold higher yield of pyrone CHS products than that obtained with the free enzyme solutions.

引用

页码：3806 / 3809

页数：4

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