Isolation and characterization of microsatellite loci from the rust pathogen, Melampsora lini

被引：6

作者：

Barrett, Luke G. ^{[1
]}

Brubaker, Curt L. ^{[1
]}

机构：

[1] CSIRO, Plant Ind, Ctr Plant Biodivers Res, Canberra, ACT 2601, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 03期

关键词：

flax; Linum marginale; Linum usitatissimum; Melampsora lini; microsatellite; rust; MARGINALE; POPULATIONS;

D O I：

10.1111/j.1471-8286.2006.01404.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed and characterized primers for 11 variable microsatellite loci present in the genome of the flax rust, Melampsora lini. The microsatellite loci were identified by sequencing clones from a library of EcoRI DNA fragments enriched for four simple sequence repeat motifs (AAG, AAT, TC and TG). All 11 primer pairs successfully amplified DNA fragments from a sample of 102 M. lini isolates (98 isolated from Linum marginale and four from Linum usitatissimum), revealing a total of 32 alleles. Allelic diversity at the 11 loci ranged from 0.030 to 0.449.

引用

页码：930 / 932

页数：3

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