Glycerol production in a triose phosphate isomerase deficient mutant of Saccharomyces cerevisiae

被引:48
作者
Compagno, C
Boschi, F
Ranzi, BM
机构
[1] Dipto. Fisiol. e Biochim. Generali, Sez. Biochimica Comparata, Univ. degli Studi di Milano, 20133 Milano
关键词
D O I
10.1021/bp960043c
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Interesting challenges from metabolically engineered Saccharomyces cerevisiae cells arise from the opportunity to obtain yeast strains useful for the production of chemicals. In this paper, we show that engineered yeast cells deficient in the triose phosphate isomerase activity are able to produce glycerol without the use of steering agents. High yields of conversion of glucose into glycerol (80-90% of the theoretical yield) and productivity (1.5 g L(-1) h(-1)) have been obtained by a bioconversion process carried out in a poor and clean medium. We obtained indications that the growth phase at which the biomass was collected affect the process. The best results were obtained using cells collected at the end of exponential phase of growth. In perspective, the strategies and the information about the physiology of the cells described here could be useful for the developing of new biotechnological processes for glycerol production, outflanking the problems related to the use of high level of steering agents.
引用
收藏
页码:591 / 595
页数:5
相关论文
共 22 条
[1]  
Agarwal G.P., 1990, ADV BIOCHEM ENG BIOT, V41, P95
[2]  
ALBER T, 1982, Journal of Molecular and Applied Genetics, V1, P419
[3]  
[Anonymous], [No title captured]
[4]  
[Anonymous], METHOD ENZYMOL
[5]  
BISPING B, 1986, APPL MICROBIOL BIOT, V23, P174
[6]   GLYCEROL PRODUCTION BY SEMICONTINUOUS FED-BATCH FERMENTATION WITH IMMOBILIZED CELLS OF SACCHAROMYCES-CEREVISIAE [J].
BISPING, B ;
HECKER, D ;
REHM, HJ .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1989, 32 (02) :119-123
[7]   MICROCALORIMETRIC MONITORING OF GROWTH OF SACCHAROMYCES-CEREVISIAE - OSMOTOLERANCE IN RELATION TO PHYSIOLOGICAL-STATE [J].
BLOMBERG, A ;
LARSSON, C ;
GUSTAFSSON, L .
JOURNAL OF BACTERIOLOGY, 1988, 170 (10) :4562-4568
[8]   PHYSIOLOGICAL-EFFECTS OF 7 DIFFERENT BLOCKS IN GLYCOLYSIS IN SACCHAROMYCES-CEREVISIAE [J].
CIRIACY, M ;
BREITENBACH, I .
JOURNAL OF BACTERIOLOGY, 1979, 139 (01) :152-160
[9]  
COMPAGNO C, 1992, APPL MICROBIOL BIOT, V36, P535
[10]   CONTINUOUS GLYCEROL PRODUCTION BY THE SULFITE PROCESS WITH IMMOBILIZED CELLS OF SACCHAROMYCES-CEREVISIAE [J].
HECKER, D ;
BISPING, B ;
REHM, HJ .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 32 (06) :627-632