The role of exogenous fibroblast growth factor-2 on the reprogramming of primordial germ cells into pluripotent stem cells

被引：81

作者：

Durcova-Hills, Gabriela ^{[1
]}

Adams, Ian R. ^{[1
]}

Barton, Sheila C. ^{[1
]}

Surani, M. Azim ^{[1
]}

McLaren, Anne ^{[1
]}

机构：

[1] CR UK Gurdon Inst Canc & Dev Biol, Wellcome Trust, Cambridge CB2 1QN, England

来源：

STEM CELLS | 2006年 / 24卷 / 06期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

reprogramming; pluripotency; fibroblast growth factors; primordial germ cells; embryonic germ cells; Chimera; mouse;

D O I：

10.1634/stemcells.2005-0424

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The germ cell lineage is a specified cell population that passes through a series of differentiation steps before giving rise, eventually, to either eggs or sperm. We have investigated the manner in which primordial germ cells (PGCs) are reprogrammed in vitro to form pluripotent stem cells in response to exogenous fibroblast growth factor-2 (FGF-2). The response is dependent on time of exposure and concentration of FGF-2. PGCs isolated in culture show a motile phenotype and lose any expression of a characteristic germ cell marker, mouse vasa homolog. Subsequently, some but not all of the cells show further changes of phenotype, accompanied by changes in expression of endogenous FGF-2 and upregulation of its receptor, fibroblast growth factor receptor-3, in the nucleus. We propose that it is from this reprogrammed component of the now heterogeneous PGC population that pluripotent stem cells arise.

引用

页码：1441 / 1449

页数：9

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